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Patrick Horn is scientist and project leader in the stem cell research group of Anthony D. Ho in the Department of Medicine V at the University of Heidelberg. His research focuses on the characterization of ageing and senescence in the hematopoietic system, especially within the stem cell niche in the bone marrow. Further specification of this niche in the human body as well as the identification of differences and causal factors of cell-interactions in a diseased environment belong to his field of interest.
Role in SyStemAge:
Access to and the processing of primary human material is one of the main contributions to the Consortium. We are involved mainly in WP1 and WP3 and have established infrastructure and methods for:
- Collection and processing of marrow specimens derived from healthy human subjects of different age groups.
- Isolation and purification of HSC as well as subsets of other cell types, e.g. MSC, macrophages, lymphocytes, etc., from cord-blood and bone marrow from donors of different age groups.
- Co-culture system of MSC and HSC as surrogate model for the hematopoietic stem cell niche of “different ages”, “diseases”, and “cross-over” experiments.
- Assays to access potential and characteristics of cell populations.
- Protocols for differential labelling (SILAC) of MSC and HSC in co-culture systems.
- Correlation with clinical data.